电离辐射通过转化生长因子-β-激活的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简要 ：聚焦放射是否可通过裂解脂质-β（TGF-β）-介导的上皮细胞-上皮细胞转换 (EMT)来促进癌细胞的侵袭迁往。使用总和2Gy(60)Coγ终点站紫外光源自生命器官的6种癌细胞，据信与EMT相关的变化，这包括分别借助显微镜技术，胺基酸马尔季尼夫卡步骤，免疫荧光技术，划痕试验里面和Transwell四人试验里面来观察并检测细胞组织形态，EMT标记，侵袭迁往技能等。采用酶亦同免疫吸附例检测这些癌细胞里面TGF-β肽水平，借助都有抗病毒SB431542来评估TGF-β信号通路在放射EMT里面的依赖性。经过总和为2Gy紫外光的癌细胞里面存在间叶细胞的表达，与假紫外光组相比其上皮细胞标记减少，间叶细胞标记增加，同时其侵袭移到技能弱化，TGF-β肽水平也提高。进一步发现由A549放射正向的EMT可通过对TGF-β信号抑制发生反败为胜。这些结果表明TGF-β介导的EMT在放射正向弱化癌细胞侵袭移到技能里面起着关键依赖性。

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